During the six days of docked operations, the joint Mir and Shuttle crews transferred more than four tons of material from the SPACEHAB Double Module to Mir.

During the flight, Wetherbee and Bloomfield fired small jet thrusters on Atlantis to provide data for the Mir Structural Dynamics Experiment (MISDE) which measures disturbances to space station components and its solar arrays. Other experiments conducted during the mission were the Commercial Protein Crystal Growth investigation; the Cell Culture Module Experiment (CCM-A), the Cosmic Radiation Effects and Activation Monitor (CREAM) and the Radiation Monitoring Experiment-III (RME-III); the Shuttle Ionospheric Modification with Pulsed Local Exhaust (SIMPLE) experiment; and the Midcourse Space Experiment. Two NASA educational outreach programs were also conducted, Seeds in Space-II and Kidsat.